Animalscaretips
- Why is the cover of the Petri dish slightly opened during inoculation of the plate?
- Are Petri dishes dangerous?
- Why do you store agar plates upside down?
- Why should you never set the lid of the petri dish or the test tube cap on the benchtop?
- What is the reasoning for leaving the lid of the agar plates at an angle while streaking the plates?
- Can you throw away Petri dishes?
- Can you grow bacteria without agar?
- Can you reuse plastic petri dishes?
- What is an agar stab?
- Does incubation time affect microbial growth?
- What is incubation condition?
- Why does the lid of the agar plate have to be removed quizlet?
- What does condensation do to agar plates?
- Is condensation in agar bad?
Why is the cover of the Petri dish slightly opened during inoculation of the plate?
Reason - The lid stops additional unwanted bacteria in the air contaminating the plate. Do not fully seal the lid, as this will stop oxygen reaching the bacterium, and this may encourage harmful anaerobic bacteria to grow.
Are Petri dishes dangerous?
Most bacteria collected in your environment will not be harmful. However, once they multiply into millions of colonies in a Petri dish they become more of a hazard. Be sure to protect open cuts with rubber gloves and never ingest or breathe in growing bacteria.
Why do you store agar plates upside down?
Petri dishes need to be incubated upside-down to lessen contamination risks from airborne particles landing on them and to prevent the accumulation of water condensation that could disturb or compromise a culture.
Why should you never set the lid of the petri dish or the test tube cap on the benchtop?
Never set them on the table, as they could pick up contaminants. ... Also, keep the lid over a plate when removing inoculum, as this will help prevent environmental contamination. Always flame the lip of a culture tube when you open it and before you replace the cap.
What is the reasoning for leaving the lid of the agar plates at an angle while streaking the plates?
Lift the lid slightly ajar and hold it at an angle, while you are streaking the plate. While this prevents contaminated dust from falling on plate, it may be difficult to see what you are doing.
Can you throw away Petri dishes?
Though you could technically throw them away as normal (bacteria are everywhere, after all), to be absolutely certain that you're not letting dangerous bacteria go, you should disinfect the dishes. You can do this with just regular bleach.
Can you grow bacteria without agar?
Plain agar won't work because it contains no 'food' for the bacteria. You have to buy NUTRIENT agar because that has sugars, salts, nitrogen source, etc that bacteria need to grow.
Can you reuse plastic petri dishes?
If your experiment requires a 100 percent pure culture and you're using plastic Petri dishes, then its best use new dishes sterilized from the manufacturer. Do not reuse plastic Petri dishes if your experiment involves the use of live pathogens. The risk of cross-contamination is too great.
What is an agar stab?
A stab is a type of Luria Broth (LB) Agar media, similar to a standard LB Agar plate. Unlike an LB Agar plate, a stab culture is created by piercing the LB agar with the bacteria instead of spreading it on the surface. ... The bacteria in the stab is guaranteed to live for at least 2 weeks when stored at 4°C.
Does incubation time affect microbial growth?
Factors such as incubation period, temperature and pH can affect the growth of biosurfactant producing bacteria, their ability to produce biosurfactant and reduce surface tension. ... tequilensis culture successfully reduced after 48 hours of incubation period.
What is incubation condition?
Microorganisms should be cultured under conditions that best support their growth including atmosphere, temperature, and duration of incubation. For example, incubation of bacteria on non-selective media for 18-48 hours, in an ambient or CO2 atmosphere, incubated at 33-37° C generally yield optimal spectra.
Why does the lid of the agar plate have to be removed quizlet?
agar plates must be kept with lid on to avoid contamination when not being used.
What does condensation do to agar plates?
If condensed water drips onto the agar, it can interfere with the growth of the colony of microorganisms. Depending on the type of experiment or study, it may be important to keep the colonies separate on a plate. Water on the agar has the potential to allow organisms to move across the plate into other areas.
Is condensation in agar bad?
Condensation does make the culture harder to see and it does look awful when you want to share that beautiful culture! For some really beautiful Agar photos and fantastic Agar tips, head over to 'Agar Of Asgard' on Facebook.
