Why do you use the same restriction enzyme when you splice together two separate things?

1. Why was it important to use the same restriction enzyme for all the DNA samples?
2. Why is it necessary that the same enzyme should be used in cutting the bacterial plasmid and gene of interest in recombinant DNA?
3. Why would different restriction enzymes generate different DNA fragments if they are all cutting the same DNA molecule?
4. Why is it useful to have many different kinds of restriction enzymes?
5. Why is it important that the same enzyme or enzymes be used to cut both the plasmid and the insulin gene from the human DNA?
6. Why is the same restriction enzyme used to cut the human chromosome and the plasmid?
7. What would happen if we used different restriction enzymes to cut the plasmid and the gene?
8. When two DNA pieces cut with the same restriction enzyme are combined sticky ends will?
9. What would happen if you cut two pieces of DNA with two different restriction enzymes?
10. What do you understand by restriction enzyme?
11. Why are restriction enzymes important in genetic engineering?
12. Why do different individuals even siblings have different restriction enzyme recognition sites?
13. What is the difference between restriction and ligation reactions?
14. Why might it be important to cut the DNA strand as closely to the desired gene as possible?
15. How does restriction endonuclease act on a DNA molecule?

Why was it important to use the same restriction enzyme for all the DNA samples?

Explanation: Restriction enzymes cut at specific sequences so the same restriction enzyme must be used because it will produce fragments with the same complementary sticky ends, making it possible for bonds to form between them. ... Their sticky ends match, and so they can be ligated together.

Why is it necessary that the same enzyme should be used in cutting the bacterial plasmid and gene of interest in recombinant DNA?

The principle is simply that, if two different DNA molecules are cut with the same restriction enzyme, both will produce fragments with the same complementary sticky ends, making it possible for DNA chimeras to form.

Why would different restriction enzymes generate different DNA fragments if they are all cutting the same DNA molecule?

What is the nucleotide sequence at which restriction enzyme cuts DNA called? Why would different restriction enzymes cut the same DNA molecule into different numbers of fragments? Each restriction enzyme cuts DNA at a different restriction site.

Why is it useful to have many different kinds of restriction enzymes?

Restriction enzymes are useful for many different applications. Because the DNA sequence is different in each organism, the pattern of restriction sites will also be different. The source of isolated DNA can be identified by this pattern.

Why is it important that the same enzyme or enzymes be used to cut both the plasmid and the insulin gene from the human DNA?

Why is it important that the same enzyme or enzymes be used to cut both the plasmid and the insulin gene from the human DNA? it is important to use the same enzyme so that both the ends of the insulin and plasmid connect. ... Each restriction enzyme cuts DNA at a specific recognition site.

Why is the same restriction enzyme used to cut the human chromosome and the plasmid?

These enzymes are important as they allow for specific genes to be cut out of a source chromosome. They also cut bacterial plasmids. Using the same restriction endonuclease enzyme to cut open the plasmid as is used to cut the gene from the chromosome results in complementary sticky ends being produced.

What would happen if we used different restriction enzymes to cut the plasmid and the gene?

Restriction endonuclease identifies and cuts the same pallindromic sequence in both DNA and Vector due to which when they will be mixed , their complementary bases will join and it will form the r-DNA, If both are cut with different RE , then on mixing they wont ligate with each other as their bases will not match.

When two DNA pieces cut with the same restriction enzyme are combined sticky ends will?

If you cut a plasmid DNA and human DNA with the same restriction enzyme, all the DNA fragments will have the same sticky ends. Combine human DNA and bacterial plasmids. The two types of DNA have the same sticky ends, so some pieces of plasmid DNA and human DNA will stick together.

What would happen if you cut two pieces of DNA with two different restriction enzymes?

You can get two different pieces of DNA to stick together if you cut them both with a restriction enzyme that makes sticky ends. The two pieces tend to attach to each other, making it possible to combine them into a recombinant DNA molecule that has DNA from two sources.

What do you understand by restriction enzyme?

A restriction enzyme is an enzyme isolated from bacteria that cuts DNA molecules at specific sequences. The isolation of these enzymes was critical to the development of recombinant DNA (rDNA) technology and genetic engineering.

Why are restriction enzymes important in genetic engineering?

Restriction enzymes are an important tool in genomic research: by cutting DNA at a specific site, they create a space wherein foreign DNA can be introduced for gene-editing purposes.

Why do different individuals even siblings have different restriction enzyme recognition sites?

Why do different individuals such as siblings have different restriction enzyme recognition sites? DNA found in each person is unique. ... Thermocycler heats and cools DNA. each round of this doubles the amount of DNA.

What is the difference between restriction and ligation reactions?

Restriction enzymes are DNA-cutting enzymes. ... DNA ligase is a DNA-joining enzyme. If two pieces of DNA have matching ends, ligase can link them to form a single, unbroken molecule of DNA. In DNA cloning, restriction enzymes and DNA ligase are used to insert genes and other pieces of DNA into plasmids.

Why might it be important to cut the DNA strand as closely to the desired gene as possible?

Why might it be important to cut the DNA strand as closely to the desired gene as possible? Cutting the DNA close to the desired gene is necessan, so that undesired sequences are left out and the sticky ends find each other.

How does restriction endonuclease act on a DNA molecule?

When they act on a DNA molecule, restriction enzymes produce "blunt" ends when they cut in the middle of the recognition sequence, and they yield "sticky" ends when they cut at the recognition sequence in a staggered manner, leaving a 5' or 3' single-stranded DNA overhang.