What is the purpose of inoculating tsa plates with samples from the zone of inhibition?

1. What was the purpose of inoculating TSA plates with samples taken from the zones of inhibition?
2. What is the importance of measuring the zone of inhibition?
3. What does MSA plate test for?
4. Why do you think it is important to do spot inoculations for the amylase test and the Caseinase test?
5. What purpose does the nutrient agar plate serve MacConkey?
6. Why it is important to remove the petri dish plate covers prior to exposing them to UV?
7. What does the Zone of Inhibition show?
8. What does the Zone of Inhibition tell you?
9. What does a zone of inhibition indicate?
10. What does MSA differentiate between?
11. What is the purpose of pea agar?
12. Why is MSA selective and differential?
13. What organism is used in the metabolism lab?
14. What purpose does nutrient agar plate serve?
15. What can be determined from inoculating bacteria on a MacConkey agar plate?
16. What does growth on MacConkey agar mean?

What was the purpose of inoculating TSA plates with samples taken from the zones of inhibition?

In the optional exercise, what was the purpose of inoculating TSA (or NA) plates with samples taken from the zones of inhibition? The purpose was to determine if the antibiotic is bacteriostatic (which should result in growth on the TSA or NA plate) or bactericidal (which should show no growth on the TSA or NA plate).

What is the importance of measuring the zone of inhibition?

A Zone of Inhibition Test, also called a Kirby-Bauer Test, is a qualitative method used clinically to measure antibiotic resistance and industrially to test the ability of solids and textiles to inhibit microbial growth.

What does MSA plate test for?

Mannitol Salt Agar (MSA) is used as a selective and differential medium for the isolation and identification of Staphylococcus aureus from clinical and non-clinical specimens. It encourages the growth of a group of certain bacteria while inhibiting the growth of others.

Why do you think it is important to do spot inoculations for the amylase test and the Caseinase test?

Why do you think it is important to do spot-inoculations for the amylase test and the caseinase test? So a large amount of bacteria will grow in a single location and produce a good amount of either enzyme. ... To do this test, why do you have to grow the organism in medium containing tryptophan?

What purpose does the nutrient agar plate serve MacConkey?

What is the purpose of the nutrient agar plate? It shows normal growth and appearance of the bacteria when grown on agar. It serves as a control for comparison to growth on MacConkey agar.

Why it is important to remove the petri dish plate covers prior to exposing them to UV?

Why do you remove the cover prior to exposing the dish to UV light? UV light does not penetrate well, and can not penetrate the plastic.

What does the Zone of Inhibition show?

The zone of inhibition is a uniformly circular zone of no bacterial growth around the antibiotic disk. The larger this zone is, the more sensitive the bacteria is to that antibiotic. The smaller the zone is, the more resistant (and, thus, less sensitive) the bacteria is.

What does the Zone of Inhibition tell you?

The Zone of inhibition is a circular area around the spot of the antibiotic in which the bacteria colonies do not grow. The zone of inhibition can be used to measure the susceptibility of the bacteria to wards the antibiotic.

What does a zone of inhibition indicate?

zone of inhibition: This is an area of media where bacteria are unable to grow, due to presence of a drug that impedes their growth. minimum inhibitory concentration: This is the lowest concentration of an antimicrobial drug that prevents visible growth of a microorganism after overnight incubation with media.

What does MSA differentiate between?

Uses of Mannitol Salt Agar (MSA)

It is used for the selective isolation and differentiation of Staphylococcus aureus from clinical samples. It is also used for the enumeration of staphylococci in food and dairy products.

What is the purpose of pea agar?

PEA agar is a selective medium that is used for the isolation of gram- positive Staphylococcus species andStreptococcus species from clinical specimens or specimens that contain mixtures of bacterial flora (2). Typically PEA agar is used to inhibit the common contaminants such as Escherichia coli and Proteus species.

Why is MSA selective and differential?

Mannitol Salt Agar (MSA) is a selective and differential medium. The high concentration of salt (7.5%) selects for members of the genus Staphylococcus, since they can tolerate high saline levels. Organisms from other genera may grow, but they typically grow very weakly.

What organism is used in the metabolism lab?

Being one of the model organisms in microbial metabolism, Lactococcus lactis has been the main target of metabolic engineering among LAB.

What purpose does nutrient agar plate serve?

Nutrient agar plates are used to grow colonies of bacteria so that their growth and development can be contained and observed in a laboratory.

What can be determined from inoculating bacteria on a MacConkey agar plate?

MacConkey agar not only selects for Gram-negative organisms by inhibiting Gram-positive organisms and yeast but also differentiates the Gram-negative organisms by lactose fermentation. ... Escherichia coli and other lactose ferments will produce yellow or orange colonies.

What does growth on MacConkey agar mean?

Result Interpretation on MacConkey Agar

Lactose fermenting strains grow as red or pink and may be surrounded by a zone of acid precipitated bile. The red colour is due to production of acid from lactose, absorption of neutral red and a subsequent colour change of the dye when the pH of medium falls below 6.8.