What is microarray?

1. What is a microarray and how does it work?
2. What does the microarray test for?
3. What is the microarray technique?
4. What is microarray in microbiology?
5. What is microarray in bioinformatics?
6. What is application of microarray?
7. What does a microarray look like?
8. Can microarray detect Williams Syndrome?
9. How is microarray made?
10. What are advantages of microarray?
11. Why is microarray so useful for Transcriptomics?
12. What is microarray PDF?
13. Why is DNA microarray an important tool?
14. What is Rnaseq data?
15. Who discovered microarray technology?
16. What is NT in DNA?
17. How is microarray analysis used today?

What is a microarray and how does it work?

A microarray is a laboratory tool used to detect the expression of thousands of genes at the same time. The DNA molecules attached to each slide act as probes to detect gene expression, which is also known as the transcriptome or the set of messenger RNA (mRNA) transcripts expressed by a group of genes. ...

What does the microarray test for?

A microarray is the recommended first line genetic test for developmental delay (DD), intellectual disability (ID) and autism spectrum disorders (ASD)*. CMA however, does not identify fragile X syndrome (FXS), a common cause of intellectual disability.

What is the microarray technique?

Microarray technology is a developing technology used to study the expression of many genes at once. It involves placing thousands of gene sequences in known locations on a glass slide called a gene chip. A sample containing DNA or RNA is placed in contact with the gene chip.

What is microarray in microbiology?

Simply defined, a microarray is a collection of microscopic features (most commonly DNA) which can be probed with target molecules to produce either quantitative (gene expression) or qualitative (diagnostic) data.

What is microarray in bioinformatics?

A “microarray” is a laboratory slide made of glass whose surface is provided with thousands of small pores in defined positions. It works under the principle of hybridization of complementary strands of DNA and enables us to analyze expressions of multiple genes in one reaction in an effective manner.

What is application of microarray?

The current scope of microarray applications includes sequencing by hybridization, resequencing, mutation detection, assessment of gene copy number, comparative genome hybridization, drug discovery, expression analysis, and immunoassay (protein microarrays).

What does a microarray look like?

The microarray is like a grid covered with thousands of tiny probes consisting of small pieces of DNA from known locations on each of the 46 chromosomes. CMA looks for imbalances of chromosomal material between DNA from a control and your patient's DNA.

Can microarray detect Williams Syndrome?

It uses a fluorescent marker to determine if the genes critical to Williams syndrome are present or not. A chromosomal microarray uses millions of markers to determine if there are missing pieces or extra pieces of DNA anywhere in the person's chromosomes.

How is microarray made?

A typical microarray experiment involves the hybridization of an mRNA molecule to the DNA template from which it is originated. Many DNA samples are used to construct an array. The amount of mRNA bound to each site on the array indicates the expression level of the various genes. This number may run in thousands.

What are advantages of microarray?

Microarray is a new powerful tool for studying the molecular basis of interactions on a scale that is impossible using conventional analysis. This technique makes it possible to examine the expression of thousands of genes simultaneously.

Why is microarray so useful for Transcriptomics?

Microarrays are particularly useful for analyzing large mammalian transcriptomes, for example in drug development and clinical research that requires rapidly assessing specific genes in thousands of samples. However, microarrays detect only known sequences, so they can't be used for discovery.

What is microarray PDF?

Microarray allows rapid measurement and visualisation of differential expression of the whole genome scale. ... The technique permits the complete analysis of genetic material and the monitoring of expression changes occurring in a biological sample under various conditions.

Why is DNA microarray an important tool?

DNA microarray is an effective tool in transcriptomics that helps us in studying and analyzing the mRNA expression of almost every gene present in an organism. With the availability of whole-genome sequencing of microorganisms, it has now become possible to identify the genes with potential for bioremediation.

What is Rnaseq data?

RNA-seq involves conversion of a sample of RNA to a cDNA library, which is then sequenced and mapped against a reference genome. In addition to the ability to measure the level of gene expression, it provides further information on alternative splicing and non-coding RNA (such as microRNA) (Chaussabel et al., 2010).

Who discovered microarray technology?

The original nucleic acid arrays were macro arrays approximately 9 cm × 12 cm and the first computerized image based analysis was published in 1981. It was invented by Patrick O. Brown.

What is NT in DNA?

nucleotide; an abbreviation of the measure of chain length of a polynucleotide.

How is microarray analysis used today?

Today, DNA microarrays are used in clinical diagnostic tests for some diseases. ... Microarrays can also be used to study the extent to which certain genes are turned on or off in cells and tissues. In this case, instead of isolating DNA from the samples, RNA (which is a transcript of the DNA) is isolated and measured.