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Why are the surface colonies on a pour plate larger than those within the medium? The colonies within the medium are surrounded by agar which places mechanical pressure on them to inhibit growth and does not permit as much exposure to air as the surface colonies.
- How do the colonies on the surface of the pour plate differ from those suspended in the agar?
- What is different about the colony growth on pour plates when compared with spread plates?
- How do the results of the pour plate method compare with those obtained using the streak plate and spread plate methods?
- What is the advantage of the pour plate method over the other methods of bacteria colony isolation What are some problems?
- Why are surface colonies on a pour plate large?
- Why is pour plate method more accurate?
- What are pour plates?
- What is the difference between a streak plate pour plate and spread plate?
- In which method isolated colonies grow only on surface of plates?
- Which are advantages of the streak plate method when compared to the pour plate method quizlet?
- Why do both surface and subsurface colonies appear in the pour plate method but not in the streak plate method to isolate pure cultures?
- What factors make the pour plate method selective?
- Why is spread plate better than pour plate?
- What is the principle of pour plate method?
How do the colonies on the surface of the pour plate differ from those suspended in the agar?
How do colonies on the surface of a pour plate differ from those suspended in the agar? If they're on the surface they are obligate aerobes- microaerophiles. If in liquid, they are obligate anaerobes -aerotolerant anaerobes. How would you determine whether a colony was a contaminant on a streak plate?
What is different about the colony growth on pour plates when compared with spread plates?
Generally, pour plates is the method for counting the number of colony-forming bacteria present in a liquid specimen. Pour plates also allow the identification of bacteria as aerobes, anaerobes or facultative aerobes. On the other hand, spread plates allow the isolation of specific clonal colonies.
How do the results of the pour plate method compare with those obtained using the streak plate and spread plate methods?
How do the results of the pour-plate method compare with those obtained using the streak-plate and spread-plate methods? The results should compare favorably with the streak plate and spread plate methods to obtain isolated colonies. The bacteria samples are diluted several times to ensure decrease # of microbes.
What is the advantage of the pour plate method over the other methods of bacteria colony isolation What are some problems?
The pour plate technique can be used to determine the number of microbes/mL in a specimen. It has the advantage of not requiring previously prepared plates, and is often used to assay bacterial contamination of food stuffs.
Why are surface colonies on a pour plate large?
Why are the surface colonies on a pour plate larger than those within the medium? More room to grow on the surface than in the agar. Toxic by-products dilute out on the surface better. More nutrients available on surface as the bacteria grows.
Why is pour plate method more accurate?
Advantages of Pour Plate Technique
Will detect lower concentrations than surface spread method because of the larger sample volume. It requires no pre-drying of the agar surface. The most common method for determining the total viable count is the pour-plate method.
What are pour plates?
Definition of pour plate
: a plate prepared by mixing the inoculum with the cooled but still fluid medium before pouring the latter into the petri dish.
What is the difference between a streak plate pour plate and spread plate?
The key difference between streak plate and spread plate is that the streak plate is used to isolate and purify a particular bacterial species from a mixture of bacteria while the spread plate is used to enumerate and quantify bacteria in a sample.
In which method isolated colonies grow only on surface of plates?
In microbiology, streaking is a technique used to isolate a pure strain from a single species of microorganism, often bacteria. Samples can then be taken from the resulting colonies and a microbiological culture can be grown on a new plate so that the organism can be identified, studied, or tested.
Which are advantages of the streak plate method when compared to the pour plate method quizlet?
What advantage does the pour-plate method have over the streak-plate method? It provides isolated colonies. It requires less materials. It only requires an inoculating tool and one plate.
Why do both surface and subsurface colonies appear in the pour plate method but not in the streak plate method to isolate pure cultures?
Why do both surface and subsurface colonies appear in the pour plate method, but not in the streak plate method to isolate pure cultures? ... We look for only colonies on the surface because if you find growth under surface, it means contamination because you didn't inoculate there.
What factors make the pour plate method selective?
Since the warm, molten agar is poured onto the bacterial suspension, the temperature could damage or kill the heat- sensitive bacteria. Thus, pour plate method is considered as selective.
Why is spread plate better than pour plate?
Pour plate is a microbial technique to enumerate a number of viable cells in a sample. ... With regard to the accuracy of these two techniques, pour plate has a higher accuracy than the spread plate. Moreover, unlike in a pour plate, a glass spreader is used to spread the sample evenly on the surface on a spread plate.
What is the principle of pour plate method?
Principle of Pour Plate Method
In this Method, serial dilutions of the inoculum (serially diluting the primary specimen) are added within sterile Petri plates to which is poured melted and cooled (42-45°C) agar medium and completely mixed by revolving the plates which are then left to solidify.
