Animalscaretips
- What color will an acid-fast organism stain?
- What is phenol carbolic acid and what is its usual application?
- How might the acid-fast characteristic of Mycobacterium?
- What diseases are diagnosed by using the acid-fast stain?
- Why is methylene blue used in acid fast staining?
- What color would bacterial cells appear if the patient has tuberculosis?
- What is the expected result from an acid fast stain of sputum from a tuberculosis patient?
- What is the correct order of the stains used in the acid fast stain procedure?
- What is cold staining?
- Which diseases are caused by acid-fast bacteria?
- What is a negative stain in microbiology?
- What type of stain should be used for staining capsules?
- What is Spore staining?
- What does an acid-fast stain tell you?
- What does a positive acid-fast stain mean?
What color will an acid-fast organism stain?
Acid fast bacteria have a high content of mycolic acids in their cell walls. Acid fast bacteria will be red, while nonacid fast bacteria will stain blue/green with the counterstain with the Kinyoun stain.
What is phenol carbolic acid and what is its usual application?
What is phenol (carbolic acid), and what is its usual application? Phenol is a disinfectant. It was the disinfectant used by Lister.
How might the acid-fast characteristic of Mycobacterium?
How might the acid-fast characteristic of Mycobacterium enhance the organism's ability to cause disease? because they have a waxy coating around their cell that prevents alot of different things from getting into the cell. Including antibiotics if a person is sick.
What diseases are diagnosed by using the acid-fast stain?
An acid-fast bacteria (AFB) culture is done to find out if you have tuberculosis (TB) or another mycobacterial infection. Besides TB, the other main mycobacterial infections are leprosy and a TB-like disease that affects people with HIV/AIDS.
Why is methylene blue used in acid fast staining?
After the auramine dye has fully stained the smear, a drop of acid alcohol is applied for one to two minutes to decolorize the smear. Methylene blue or potassium permanganate is used as a counterstain to provide background color.
What color would bacterial cells appear if the patient has tuberculosis?
The purple rod-shaped organism is a TB bacterium.
What is the expected result from an acid fast stain of sputum from a tuberculosis patient?
Sputum, or phlegm, is often used to test for Mycobacterium tuberculosis, to find out if a patient has TB. This bacterium is completely acid-fast, which means the entire cell holds onto the dye. A positive test result from the acid-fast stain confirms the patient has TB.
What is the correct order of the stains used in the acid fast stain procedure?
The correct order of an acid-fast stain is primary, decolorizing, counterstaining.
What is cold staining?
The cold staining method is simple, because it eliminates heatingof stain. For direct smear, the correlations of cold staining procedure with conventional ZN method was 93% and for post concentration smear it was 100%. KEY WORDS: Acid fast bacilli, Mycobacterial cold staining.
Which diseases are caused by acid-fast bacteria?
Acid-fast bacillus (AFB) is a type of bacteria that causes tuberculosis and certain other infections. Tuberculosis, commonly known as TB, is a serious bacterial infection that mainly affects the lungs. It can also affect other parts of the body, including the brain, spine, and kidneys.
What is a negative stain in microbiology?
In microscopy, negative staining is an established method, often used in diagnostic microscopy, for contrasting a thin specimen with an optically opaque fluid. In this technique, the background is stained, leaving the actual specimen untouched, and thus visible.
What type of stain should be used for staining capsules?
Bacterial capsules are non-ionic, so neither acidic nor basic stains will adhere to their surfaces. Therefore, the best way to visualize them is to stain the background using an acidic stain and to stain the cell itself using a basic stain. We use India ink and Gram crystal violet.
What is Spore staining?
Endospores staining is the type of staining to recognize the presence spore in bacterial vegetative cells. The bacterial endospores need a staining which can penetrate wall thickness of spore bacteria. ... It is an alkaline substance staining that can staining the spore bacteria.
What does an acid-fast stain tell you?
The acid-fast stain is a laboratory test that determines if a sample of tissue, blood, or other body substance is infected with the bacteria that causes tuberculosis (TB) and other illnesses.
What does a positive acid-fast stain mean?
A normal result for an acid-fast bacteria smear is negative, meaning no bacteria were found in the sputum sample. A positive result means that bacteria were found and that you may have an infection. The smear is treated with a special acid-fast stain that can provide a preliminary test result in 24 hours.
