What are the advantage and disadvantages of fresh specimen?

1. What are the advantages of fresh specimens?
2. What are the advantages and disadvantages of fresh specimen and permanent slides?
3. What is the disadvantage of fresh tissue examination?
4. What objective is used when observing fresh specimen?
5. What is a fresh specimen?
6. Why is staining important in fresh specimen preparation?
7. What are the advantages of permanent slides?
8. What are the advantages of a prepared slide?
9. What is the advantage of making a temporary mount of cells?
10. What is fresh tissue?
11. What are the different methods of preparing fresh tissues for microscopic examination?
12. What are the methods of preparing fresh tissues for microscopic examination?
13. Why do we need to prepare specimens?
14. How important is microscope in studying specimen?
15. Why it is important to fix a specimen before viewing it under a light microscope?

What are the advantages of fresh specimens?

ADVANTAGES OF FRESH SPECIMEN

Fresh specimens are harder to store than preserved. They also can decompose quickly so you have to work fast. If they decompose before you collect your data, your experiment may be jeopardized.

What are the advantages and disadvantages of fresh specimen and permanent slides?

Over time the specimen may also start to lose color. Generally, permanent slides require much more elaborate preparation. The advantage is, however, that once prepared the slide can be used over and over again and can be stored for longer time periods.

What is the disadvantage of fresh tissue examination?

Its use has been limited, however, because of the fact that tissues examined in the fresh state are not permanent, and therefore, are liable to develop to develop the changes that have usually been observed after death. ...

What objective is used when observing fresh specimen?

Fixation is a crucial step in preparing specimens for microscopic examination. Its objective is to prevent decay and preserve cells and tissues in a “life-like” state.

What is a fresh specimen?

Test Name Fresh Specimens Specimen Type. Tissue that is required to be sent to the laboratory fresh for further testing such as frozen section, micro/culture, flow cytometry or karyotyping.

Why is staining important in fresh specimen preparation?

The main reason you stain a specimen before putting it under the microscope is to get a better look at it, but staining does much more than simply highlight the outlines of cells. Some stains can penetrate cell walls and highlight cell components, and this can help scientists visualize metabolic processes.

What are the advantages of permanent slides?

Permanent preparations of particles on microscope slides can be made in any number of ways. Advantages to using this method include fewer trapped air bubbles, less chance of sample loss, less chance of contaminating the mounting medium and less waste of mounting medium.

What are the advantages of a prepared slide?

In addition to helping learners to identify or observe given specimens, prepared microscope slides increase available viewing options by expanding far beyond your local environment depending on where you live. There are typically a wide variety of slides with a range of exotic and rare specimens.

What is the advantage of making a temporary mount of cells?

Advantages and disadvantage of a wet mount

Compared to permanently mounted slides, wet mounts do have certain advantages: Quick preparation: specimen fixation, dehydration and staining are not necessary (but possible, if required). For this reason, wet mounts are the first kind of mounts that students learn to make.

What is fresh tissue?

Similar to FFPEs and frozen samples, fresh tissue collections are procured under IRB approval as a surplus during surgical interventions. In the case of fresh tissue collections, as the name implies, there is no fixation of the tissue, making this the format of choice for primary cell isolations or 3D cultures.

What are the different methods of preparing fresh tissues for microscopic examination?

There are four steps in tissue preparation. Fixation stabilizes and preserves the tissue. Embedding converts the tissue into a solid form which can be sliced ("sectioned"). Sectioning (slicing) provides the very thin specimens needed for microscopy.

What are the methods of preparing fresh tissues for microscopic examination?

In a process for preparing tissue for microscopic examination which process includes the steps of fixing a specimen of tissue, dehydrating the fixed specimen, clearing the dehydrated specimen, and impregnating the cleared specimen with paraffin, ultrasonic energy is applied to the specimen in each of the processing ...

Why do we need to prepare specimens?

Specimen preparation is important in any microscopical technique with proper preparation methods facilitating examination and interpretation of microstructural features. ... This polishing stage is necessary to remove cutting and grinding damage, and to expose an unaltered cross section of the material's microstructure.

How important is microscope in studying specimen?

The benefit of the microscope is that it produces a better resolution than the naked eye. The higher the resolution, the better is our ability to detect two distinct objects without any blurring of the image. As the technology advances, the resolution becomes better [7].

Why it is important to fix a specimen before viewing it under a light microscope?

Fixation is often achieved either by heating (heat fixing) or chemically treating the specimen. In addition to attaching the specimen to the slide, fixation also kills microorganisms in the specimen, stopping their movement and metabolism while preserving the integrity of their cellular components for observation.