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DNA microarray is an effective tool in transcriptomics that helps us in studying and analyzing the mRNA expression of almost every gene present in an organism. With the availability of whole-genome sequencing of microorganisms, it has now become possible to identify the genes with potential for bioremediation.
- What is the importance of DNA microarray?
- What is the purpose of performing a DNA microarray quizlet?
- Why is microarray so useful for Transcriptomics?
- What is application of microarray?
- What is a DNA microarray quizlet?
- How are DNA microarrays used quizlet?
- Which of the following results can a DNA microarray assay detect or identify quizlet?
- What is DNA microarray technology?
- What is microarray testing?
- How microarray can be used for gene expression profiling?
- What is microarray in bioinformatics?
- How does microarray technology help in diagnosis of disease?
- What are the major steps in preparing a microarray experiment?
- What would a yellow green spot on the microarray mean quizlet?
- What is in vitro gene therapy?
What is the importance of DNA microarray?
The DNA microarray is a tool used to determine whether the DNA from a particular individual contains a mutation in genes.
What is the purpose of performing a DNA microarray quizlet?
To view and interpret at one time, on one small surface, the extent to which thousands of genes have been expressed in cells.
Why is microarray so useful for Transcriptomics?
Microarrays provide the distinct advantage of assaying millions of distinct sequences in parallel which makes the technique immune to issues detecting and measuring low abundance transcripts, or rare alternative splicing events.
What is application of microarray?
The current scope of microarray applications includes sequencing by hybridization, resequencing, mutation detection, assessment of gene copy number, comparative genome hybridization, drug discovery, expression analysis, and immunoassay (protein microarrays).
What is a DNA microarray quizlet?
What is DNA microarray? ... A technique in which single-stranded nucleic acids (DNA/RNA) are allowed to interact so that complexes called hybrids are formed by molecules with similar, complementary sequences.
How are DNA microarrays used quizlet?
A DNA microarray is used to detect and measure the expression of thousands genes at one time. ... These fragments, ideally representing all the genes of an organism, are tested for hybridization with various samples of cDNA molecules.
Which of the following results can a DNA microarray assay detect or identify quizlet?
Which of the following results can a DNA microarray assay detect or identify? They allow the expression of many or even all of the genes in a genome to be compared at once. Researchers are looking for better treatments for breast cancer.
What is DNA microarray technology?
Microarray technology is a developing technology used to study the expression of many genes at once. It involves placing thousands of gene sequences in known locations on a glass slide called a gene chip. A sample containing DNA or RNA is placed in contact with the gene chip.
What is microarray testing?
“Microarray” refers to a microchip-based testing platform that allows high-volume, automated analysis of many pieces of DNA at once. CMA chips use labels or probes that bond to specific chromosome regions. Computer analysis is used to compare a patient's genetic material to that of a reference sample.
How microarray can be used for gene expression profiling?
Microarray technology is capable of simultaneously measuring the expression levels of thousands of genes in a biological sample at the mRNA level. ... When fluorescence-labelled cDNA is hybridised to these arrays, expression levels of each gene in the human genome can be quantified using laser scanning microscopes.
What is microarray in bioinformatics?
A “microarray” is a laboratory slide made of glass whose surface is provided with thousands of small pores in defined positions. It works under the principle of hybridization of complementary strands of DNA and enables us to analyze expressions of multiple genes in one reaction in an effective manner.
How does microarray technology help in diagnosis of disease?
Diagnostic DNA microarrays have been used for genotyping and determination of disease-relevant genes or agents causing diseases, mutation analysis, screening of single nucleotide polymorphisms (SNPs), detection of chromosome abnormalities, and global determination of posttranslational modification.
What are the major steps in preparing a microarray experiment?
There are three major steps involved in a typical experiment involving a microarray: preparation of microarrays; preparation of fluorescently labeled cDNA probes and hybridization; and finally scanning, image and data analysis.
What would a yellow green spot on the microarray mean quizlet?
A green spot indicates that that gene was strongly repressed in cancer cells. (In your experiment these spots will be light pink.) • If a spot turns yellow, it means that that gene was neither strongly expressed nor strongly repressed in cancer cells. (In your experiment these spots will be clear.)
What is in vitro gene therapy?
In vivo gene therapy means that therapy is administered directly the patient. The targeted cells remain in the body of the patient. With ex vivo gene/cell therapy the targeted cells are removed from the patient and gene therapy is administered to the cells in vitro before they are returned to the patient's body.
